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. 2018 Sep 20;12:644. doi: 10.3389/fnins.2018.00644

FIGURE 5.

FIGURE 5

Glycine-histidine-lysine (GHK) decreases miR-339-5p levels via the p38 pathway, while miR-339-5p overexpression prevents GHK-induced increases in VEGFA expression in SH-SY5Y cells treated with hemin. (A) Expression of phosphorylated p38 in GHK-treated SH-SY5Y cells. SH-SY5Y cells were incubated with 1 μM or 10 μM GHK. (B) Expression of miR-339-5p in SH-SY5Y cells stimulated with GHK and p38 activator. Cells were treated with GHK (10 μM) and different concentrations of the p38 activator dehydrocorydaline. Levels of miR-339-5p were detected 24 h after treatment. (C) Expression of VEGFA in SH-SY5Y cells stimulated with GHK and p38 activator. Cells were treated with GHK (10 μM) and different concentrations of the p38 activator dehydrocorydaline. Levels of miR-339-5p were detected 48 h after treatment. (D) The apoptotic rate of SH-SY5Y cells. SH-SY5Y cells were stimulated with GHK and transfected with miR-339-5p. (Data are shown as the mean ± SD, n = 3 and ∗p < 0.05.)