FIGURE 1.
Expression pattern of TpNRAMP3. (A) At different growth stages, the relative expression of TpNRAMP3 in various tissues that were collected from DPW grown in a field at natural wheat growth season (B,C): the relative expression of TpNRAMP3 in roots (B) and shoots (C) that treated with Mg, Zn, Fe, Cu, Cd, Pb, or Ni for 24 h. Asterisk indicated significant difference from control at P < 0.05 by Tukey’s test with three independently biological replicates. Jointing stage: the basal stem emerges above the soil line; booting stage: the developing head with the sheath of the flag leaf becomes visibly enlarged; internode I: the first internode below the spike; internode II: the second internode below the spike; node I: the node with the flag leaf; node II: the first node below the node I; node III: the second node below the node I. CK was treated without supplementation with metals.