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. 2018 Sep 20;9:548. doi: 10.3389/fendo.2018.00548

Table 1.

Quantitative reverse transcription polymerase chain reaction (RT-qPCR) validation of RNA-seq genes for selected differentially expressed genes.

Fold change
Gene qPCR primers Prenatal exposure Postnatal exposure
RNA-seq qPCR RNA-seq qPCR
Cd36 TGTGTTTGGAGGCATTCTCA
TTTGAAAGCAGTGGTTCCTTC
2.25 2.01 5.58 4.99
Abcd2 TGTGGAGCAGCTGTGGACTA
CATAGCCTGCTTTGGACCAT
11.00 9.76 5.90 5.37
Prelid2 TGTTCCAGTACCCCTTCGAG
TTCCACAGTTTTTACAGAGATGACA
−5.28 −6.11 −5.13 −6.20
Apoa4 AGTGAGGAGCCCAGGATGTT
CACCTGGTCCGAAGTGACCT
−3.48 −2.98 −3.81 −3.45
Fabp4 AATGTGTGATGCCTTTGTGG
CACTTTCCTTGTGGCAAAGC
−6.68 −5.34 −6.06 −7.01
Fgl1 GTGGATGGACTGAGCCTAGC
TTCCCATTCTTCCCACTGAG
−2.41 −2.55 −6.59 −5.17
Gdpd3 CCTTTTGTCTCCATCCCTGA
CCACAGCGAAATGGGAAGTA
16.91 11.39 6.23 5.86
Hao2 GAGGCAGCTTGATGAGGTTC
CCCACCATCCATGTACACTTC
4.86 5.12 7.73 6.03
B2m CCGGCCTGTATGCTATCCAG
TGTTCGGCTTCCCATTCTCC
Housekeeping gene