Table 2.
Gene set enrichment analysis (GSEA) of ribosome dataset in transcriptomic studies of PBDE effect in rodents and details of experimental design of these studies.
| Model | Sex | Tissue | Exposure chemical | Daily dose, mg/kg/body weight | Exposure duration (ED–embryonic day, (PND–postnatal day) | PND for outcome | Gene expression analysis approach | Normalized enrichment score/Nominal p-value KEGG*ribosome | Source |
|---|---|---|---|---|---|---|---|---|---|
| Rat | Male | liver | DE-71** | 50 | ED6-PND22 | 22 | Affymetrix Rat Genome 230 2.0 Array | −2.14/0.000 | (45) |
| Rat | Female | liver | DE-71** | 50 | ED6-PND22 | 22 | Affymetrix Rat Genome 230 2.0 Array | −1.98/0.000 | (45) |
| Rat | Male | liver | DE-71** | 50 | ED6-PND91 | 91 | Affymetrix Rat Genome 230 2.0 Array | −1.97/0.000 | (45) |
| Rat | Male | liver | BDE-47 | 0.2 | ED15-PND21 | 27 | Illumina BeadChips RatRef-12 | 1.39/0.034 | (43) |
| Rat | Female | brain frontal lobes | BDE-47 | 0.2 | ED15-PND21 | 41 | Illumina BeadChips RatRef-12 | 1.40/0.033 | (44) |
| CD1 mice | Male | liver | BDE-47 | 0.2 | ED8-PND21 | 21 | Illumina TruSeq RNA-Seq | 1.60/0.006 | (18) |
| CD1 mice | Male | liver | BDE-47 | 0.2 | ED8-PND21 | 140 | Illumina TruSeq RNA-Seq | 3.21/0.000 | (18) |
| CD1 mice | Male | liver | BDE-47 | 1 | ED8-ED21 | 300 | Illumina TruSeq RNA-Seq | −2.49/0.000 | Current study |
| CD1 mice | Male | liver | BDE-47 | 1 | PND1-PND21 | 300 | Illumina TruSeq RNA-Seq | −1.59/0.019 | Current study |
*
KEGG, Kyoto Encyclopedia of Genes and Genomes.
**
DE-71 is a commercial mix of PBDE, which includes primarily the tetra- through penta-PBDEs and a small component of hexa-BDE.