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. 2018 Sep 20;12:651. doi: 10.3389/fnins.2018.00651

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Copyright © 2018 Rabesandratana, Goureau and Orieux.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative images of human iPS cell-derived RGCs by adapting protocol from Reichman et al. (2017). (A) Representative image of a self-organized 8-week-old 3D retinal organoid (B) Retinal cells dissociated from 8-week-old 3D retinal organoids observed using phase contrast microscopy. (C) Immunostaining on retinal cells dissociated from 8-week-old 3D retinal organoids reveal the expression of neuronal markers (THY1, TUJ1), retinal progenitor cell-associated marker (PAX6) and RGC-associated marker (BRN3A). Nuclei were counterstained by DAPI (blue). Scale bars: 100 μm.