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. 2018 Sep 20;6:117. doi: 10.3389/fcell.2018.00117

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Copyright © 2018 Blechman, Anbalagan, Matthews and Levkowitz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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cnga2 isoforms genome editing using CRISPR/Cas9. (A) Schematic representation of the genetic structure of cnga2 isoforms. cnga2a and cnga2b. (B) Schematic representation of the predicted CNGA2a and CNGA2b protein products following sequence analysis of germline transmitted CRISPR-induced mutation alleles. (C) Embryos and adult fish were screened by PCR for germline transmission using gene-specific primers. PCR products were resolved in 1% LE agarose gels for cnga2a-del and cnga2b-del progenies and in 4% MetaPhor gel for cnga2a-stop and cnga2b-stop progenies.