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. 2018 Sep 20;6:117. doi: 10.3389/fcell.2018.00117

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Copyright © 2018 Blechman, Anbalagan, Matthews and Levkowitz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-CNGA2a immunoreactivity is not affected following KO of cnga2a and/or cnga2b. (A) Immunostaining and confocal imaging of 6-day-old larva NPO of the wild type zebrafish (n = 30/30), cnga2a (n = 20/20), cnga2b (n = 16/16), and double cnga2a/2b (n = 6/6) zebrafish mutant variants with anti-CNGA (magenta) and anti-OXT (gray scale) antibodies. (B) Anti-CNGA2a mAb immunostaining and confocal imaging of the hypothalamus and neurohypophysis of 6-day-old larva injected with different concentrations of cnga2a missense morpholino oligonucleotide (n = 10/10). Scale bars: 10 μm.