Figure 2.

Multiple types of microglial process contacts onto axonal segments were observed following brain injury. Representative 3D reconstructions of APP+ injured axonal swellings (green) and Iba-1+ microglia (white). (A) Microglial processes that terminated on the axonal surface were considered “terminal processes” (purple arrow heads). (B) Processes that continued past the axon were considered “process passes” (purple arrow). (C) Processes that encircled the axon were considered “process cups.” This nomenclature and identification scheme was used for all assessments of microglial physical interactions with axons in rats and micro pigs throughout the study.