Figure 5.

The combination of PD1, GITR, and radiation requires CD4+ T cells and partially CD8+ T cells. Mice were inoculated with primary and secondary tumors and were treated with XRT, anti-PD1, and anti-GITR, similar to previous experiments. Starting on day 6 post inoculation, depleting antibodies were administered, initially with a bolus of 500 ug and then once weekly with 200 ug. Anti-GITR therapy started on day 7 followed by XRT and PD1 on day 8. (A) Primary and (B) secondary tumors are shown from the groups (n = 7). Gating strategy seen in Supplementary Figure 2.