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. 2018 Sep 20;9:2230. doi: 10.3389/fmicb.2018.02230

FIGURE 4.

FIGURE 4

Reactivity by iELISA of MAbs against cell envelope antigens with B. ovis PA and selected mutants. The reactivity of MAbs specific against PG, R-LPS, the BP26 periplasmic protein, the Omp2b, Omp31, and Omp25 major OMPS, and the Omp10, Omp16, and Omp19 OM lipoproteins (A–F) was tested with most of the mutant strains obtained in this work. Representative results are shown in panels A–F and others are commented in the text. Differences between the mutant strains and the parental strain were considered relevant for further analysis when, in addition to being statistically significant (P < 0.05), the mean values differed more than 25% (marked with an asterisk). Reactivity of Δomp31 single and multiple mutants with several anti-Omp25 MAbs (G), and of Δomp19 mutants with several anti-BP26 MAbs (H), is also shown. Statistically significant differences between the mutant strains and the parental strain (P < 0.05) in panels G and H, are marked with an asterisk.