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. 2018 Sep 20;12:637. doi: 10.3389/fnins.2018.00637

FIGURE 4.

FIGURE 4

Protein expression of BDNF, NGF, and NTF3 after chronic fluoxetine administration in the hippocampus and PFC of wild-type, Creb1TPH2CreERT2 and Creb1TPH2CreERT2Crem–/– mice. Chronic fluoxetine treatment (10 mg/kg, ip, 1x daily, 21 days) induced BDNF upregulation in the hippocampus of male and female mice (B) and in the PFC of female (F) wild-type animals; the effect was abolished in Creb1TPH2CreERT2 mice (B,F). Fluoxetine increased NGF (G) and NTF3 (H) levels in the PFC of male Creb1TPH2CreERT2 mutants. Western blot analyses of the effects of fluoxetine administration on the protein levels of (B,F) BDNF, (C,G) NGF, and (D,H) NTF3 in the hippocampus (left panel) and the PFC (right panel) of wild-type, Creb1TPH2CreERT2, and Creb1TPH2CreERT2Crem–/– mice. (A,E) Representative blots of BDNF, NGF, NTF3 and GAPDH in saline (wells 1–3)- and fluoxetine (wells 4–6)-treated wild-type (wells 1, 4), Creb1TPH2CreERT2 (wells 2, 5) and Creb1TPH2CreERT2Crem–/– (wells 3, 6) mice. Data are presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01 vs. saline-injected wild-type mice of the same sex. W/t, wild-type; SAL, saline; FLX, fluoxetine.