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. 2018 Sep 20;9:399. doi: 10.3389/fgene.2018.00399

FIGURE 1.

FIGURE 1

Comparison of the four BnFAD2 groups and partial alignments of BnFAD2-1 and BnFAD2-2. (A) Sequence comparison highlighting the differences between BnFAD2-1, BnFAD2-2, BnFAD2-3, and BnFAD2-4. The white box in BnFAD2-3 indicates the missing 15-bp nucleotide sequence; the differently shaded boxes at 41–46 denote nucleotide difference. The BnFAD2 homolog genes have characteristic nucleotides at sites 732 and 735. ATG is the start codon; TGA, the stop codon. (B) Amino acid sequence alignment of the BnFAD2-1 and BnFAD2-2 peptides. The mutant amino acids are bracketed in square frames. The numbers represent the number of residues from the initiation amino acid Met. JN992606 and JN992607 were used as representative sequences (downloaded from GenBank). wt, wild-type; mut, mutant.