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. 2018 Sep 20;9:399. doi: 10.3389/fgene.2018.00399

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Copyright © 2018 Long, Hu, Gao, Chen, Zhang, Cheng and Pu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genotyping of the segregation lines using CAPS markers. The genotype of each line is shown under its enzyme-digested band. The left panel shows the band types for the P1, P2, F1, and F2-1 populations digested by BssS^αI (upper) and HinfI (lower) restriction enzymes, and no. 1–20 refer to the single lines from the F2-1 population. The right panel shows the band types for the P1, P2, F1, and BC1-1 populations digested by BssS^αI (upper) and HinfI (lower) restriction enzymes, and no.1-9 refer to the single lines from the BC1-1 population. M, DNA marker; P1, mutant line N1379T; P2, normal line 16wh53. The 60, 80, and 58 bp bands produced by HinfI appear as a single band on the gel because of their very similar lengths; however, these could be differentiated during our analysis. There were three standard bands in the M lane representing the 750, 500, and 250 bp band sizes.