FIGURE 4.

Enantioselective identification of the product of EeBOS. The product of the in vitro enzymatic assay with FPP were analyzed by GC-MS with a cyclodextrin column (solid black line). To confirm the enantiomer the EeBOS sample was compared with pure standards of [(–)-α-bisabolol; dotted black line], a known (+)-epi-bisabolol (L. dulcis extracts; dotted gray line), a known (+)-α-bisabolol (extract of N. benthamiana expressing A. annua bisabolol synthase; solid gray line). On the left side of both panels are the MS spectra for the (–)-α-bisabolol standard and EeBOS the in vitro enzymatic assay with FPP.