FIG 5.

Levels of total HIV DNA and cell-associated HIV RNA in CD32⁺ and PD-1⁺ CD4 T cell populations. (A) Gating strategy for CD32⁺ and CD32⁻ and PD-1⁺ and PD-1⁻ memory (CD45RA⁻) CD4 T cell populations. (B) Quantification of total HIV DNA (copies per million cells) of sorted memory (CD45RA⁻) CD4 T cells expressing or not expressing CD32 and PD-1 from 7 aviremic ART-treated (round circles) and 7 viremic (squares) individuals. (C) Frequencies of cells with inducible replication-competent HIV in sorted CD32⁻, PD-1⁻, and total memory CD4 T cells estimated using conventional limiting-dilution methods by extreme limiting-dilution analysis (http://bioinf.wehi.edu.au/software/elda/) in 3 aviremic ART-treated individuals. (D) Gating strategy for CD32⁻ PD-1⁻, CD32⁺ PD-1⁻, CD32⁻ PD-1⁺, and CD32⁺ PD-1⁺ memory (CD45RA⁻) CD4 T cell populations. (E) Levels of cell-associated unspliced HIV RNA (copies/million cells) in sorted CD32⁻ PD-1⁻, CD32⁺ PD-1⁻, CD32⁻ PD-1⁺, and CD32⁺ PD-1⁺ memory CD4 T cell populations isolated from 10 aviremic ART-treated and 6 viremic individuals. *, P < 0.05; **, P < 0.01; ns, not significant (values were obtained by a Wilcoxon signed-rank test, and error bars denote means ± SEM).