FIG 3.

Analysis of HIV-1 DNA synthesis and nuclear entry in cells depleted for components of the dynein-dynactin-BICD2 complex. TZM-bl cells were pretreated with individual siRNA duplexes for 72 h and then inoculated with GFP-expressing HIV-1 pseudotyped with VSV-G. Cells were harvested for quantitative analysis of 2-LTR circles (A) and for analysis of second strand transfer reverse transcripts (B). The effects relative to nontargeting siRNA treatment are shown. The results shown are mean values from three independent determinations. Error bars represent standard deviations. Statistical significance was calculated by an unpaired parametric t test for each siRNA treatment versus nontargeting control siRNA treatment (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001). No significant effects of the siRNAs on late reverse transcripts were observed.