FIG 4.

Effects of depletion of components of the dynein-dynactin-BICD2 complex on MLV and SIV infection. (A to E) TZM-bl cells were pretreated with individual siRNA duplexes for 72 h and inoculated with GFP-expressing HIV-1 (NL43-GFP), as well as VSV-G-pseudotyped HIV-1 (HIV-GFP), murine leukemia virus (MLV-GFP), and simian immunodeficiency virus (SIV-GFP). Inoculated cells were fixed and analyzed by fluorescence-activated cell sorting for GFP expression, and the extent of infection relative to nontargeting siRNA treatment was determined. Shown are mean values and standard deviations from three independent experiments. The statistical significance was calculated by an unpaired parametric t test for each siRNA treatment compared to nontargeting control siRNA treatment (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).