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. 2018 Sep 26;92(20):e01243-18. doi: 10.1128/JVI.01243-18

FIG 11.

FIG 11

Effect of substitutions outside the 2nd SIA-binding site on NA enzymatic activity. Specific activities of wild-type and mutant HN determined by MUNANA assay (A) or by ELLA using fetuin (B) or transferrin (C) in combination with lectin ECA were graphed normalized to the activity of wild-type HN. Means of at least two independent experiments performed in triplicate are shown. Standard deviations are indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001.