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Substitution N369H affects H5N1 NA enzymatic activity. Specific activities of the indicated (mutant) H5N1 NA proteins determined by MUNANA assay (A) or by ELLA using fetuin (B) or transferrin (C) in combination with lectin ECA were graphed normalized to the activity of N1 HN. Means of two independent experiments performed in triplicate are shown. Standard deviations are indicated.
