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Effect of substitutions in the 2nd SIA-binding site on NA enzymatic activity. Specific activities of wild-type and mutant HN determined by MUNANA assay (A) or by ELLA using fetuin (B) and transferrin (C) in combination with lectin ECA were graphed normalized to that of wild-type HN. Means of 2 to 3 independent experiments performed in triplicate are shown. Standard deviations are indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
