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Plaque formation by hybrid viruses expressing VACV and MYXV intermediate transcription factors. (A) Diagram of viruses expressing the VACV WRA8 and WRA23 (WT), MYXA8 and WRA23 (MYXA8), and WRA8 and MYXA23 (MYXA23). (B) Plaques were stained with crystal violet at 48 h after infection of BS-C-1 cells with WT or three independent clones of the hybrid viruses labeled A, B, and C.
