TABLE 2.

Mutation abundance in MYXA8 P10 passage population and clones

Passage series	Amino acid change	ORF	Gene	Mutation frequency (%)
				Passage populationa	Clone populationb
A	M424I	J6R	RPO147e	30	100
	L013M	MYXA8c	MYXA8	20	100
B	A1212V	J6R	RPO147	6	100
	None	MYXA8d	MYXA8	18	100
	A1026V	A24R	RPO132	14	0
	A1133T	A24R	RPO132	30	0
C	K1046Q	A24R	RPO132	69	100

^aPassage 10 virus was amplified prior to DNA purification.

^bVirus from large plaques was clonally purified and amplified.

^cNew translation initiation codon within N-terminal V5 tag preceding MYXA8 ORF.

^dImproved Kozak consensus sequence preceding N-terminal V5 tag; carries a single nucleotide change at the −3 position relative to the start codon.

^eRPO, RNA polymerase subunit.