FIG 1.

Fluorescent imaging of capsids, gB, and amyloid precursor protein (APP) in CAD neurons. Differentiated CAD neurons growing on poly-lysine–laminin-coated glass coverslips were infected for 22 h with a baculovirus expressing APP-BFP, a cargo transported by kinesin-1 proteins. The neurons were subsequently infected with HSV recombinant GS2843, which expresses gB-GFP and VP26-RFP (small capsid protein), for 18 h before being fixed with 4% PFA and imaged. The right side of the image shows a neuron with a cell body (labeled), which was filled with capsids and extended an axon toward the left over top of an uninfected neuron with a cell body that showed no capsids in the nucleus (uninfected cell). Arrowheads in panels A and B indicate enveloped virions decorated with VP26-RFP (capsids in panel A) and gB-GFP (glycoproteins and virion envelope in panel B) in the infected CAD neuronal axon. Panel C shows APP-BFP puncta in the same axon, with Arrowheads pointing to puncta that also showed VP26-RFP and gB-GFP fluorescence. Most APP⁺ vesicles contained HSV enveloped particles as shown in panel D, which represents the merging of GFP, RFP, and BFP signals. The inset in panel D is a magnified image showing colocalization of APP-BFP with VP26-RFP and gB-GFP signals indicated with arrows.