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Imaging of fluorescent growth-associated protein 43 (GAP43) and capsids in CAD neuron axons. CAD neurons were infected for 22 h with a baculovirus expressing GAP43-GFP, a cargo molecule transported by kinesin-1 family members, and then infected with GS2822, which expresses VP26-RFP (small capsid protein fused to RFP), for 18 h. The neurons were fixed with 4% PFA and imaged. Arrows indicate sites of colocalization of VP26-RFP containing capsids with GAP43-GFP puncta in the axon.
