FIG 4.

Imaging of fluorescent Rab5a-GFP and Rab7a-GFP with HSV capsids in axons. CAD neurons were infected for 22 h with baculoviruses expressing Rab5a-GFP or Rab7a-GFP (inset), which are both endosomal markers and cargo molecules that are transported by kinesin-3 family members. Neurons were subsequently infected with GS2822, which expresses VP26-RFP, for 18 h and then fixed with 4% PFA. There was little or no colocalization of Rab5a-GFP or Rab7a-GFP with VP26-RFP containing capsids within axons.