FIG 5.

Imaging of fluorescent kinesin KIF5C and HSV capsids in axons. CAD neurons were infected for 22 h with a baculovirus expressing kinesin KIF5C tail-GFP, a kinesin heavy-chain 1 (KIF5C) protein in which the motor domain was replaced by GFP sequences. Subsequently, the neurons were infected for an additional 18 h with GS2822, which expresses VP26-RFP, and then fixed with 4% PFA. Arrows indicate colocalization of KIF5C tail-GFP and VP26-RFP containing capsids within an axon. The kinesin-1 KIF5C tail extensively colocalized with HSV particles.