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Imaging of APP and KIF5C tail in axons. CAD neurons were infected with two baculoviruses, one expressing APP-BFP and a second expressing KIF5C tail-GFP, for 22 h and then fixed with 4% PFA and imaged. Arrows indicate colocalization of APP-BFP and KIF5C tail-GFP within an axon. The fluorescent kinesin-1 cargo APP was extensively colocalized with fluorescent kinesin-1 KIF5C tail.
