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Imaging of fluorescent KIF1A and HSV capsids in axons. CAD neurons were infected for 22 h with a baculovirus expressing KIF1A-GFP (a kinesin-3 family member) and then infected with GS2822, expressing VP26-RFP, for 18 h. The neurons were fixed with 4% PFA and then imaged. Inset 1 shows a more congested region of the axon that has many capsids and fewer KIF1A puncta, and inset 2 shows a less congested region of the axon. There was little colocalization of KIF1A-GFP with VP26+ HSV capsids.
