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Fluorescent imaging of CgA and KIF1A in CAD neurons. CAD neurons were infected with two different baculoviruses: one expressing CgA-RFP, a cargo transported by kinesin-3 family members, and one expressing KIF1A, a kinesin-3 family member, for 20 h. The neurons were then fixed with 4% PFA. Arrows indicate colocalization of CgA-RFP and KIF1A-GFP in the axon. The kinesin-3 cargo CgA-RFP extensively colocalized with the kinesin-3 KIF1A-GFP.
