TABLE 1.

List of cargo molecules expressed as fluorescent proteins using baculoviruses

Cargoa	Fluorescent tag	Expressionb	Transport into axonsc	Diffuse or punctate localization in axonsd
APP-BFP	BFP	Yes	Yes	Punctate
BDNF-BFP	BFP	Yes	Yes	Punctate
SNAP25-GFP	GFP	Yes	Yes	Punctate
VAMP1-BFP	BFP	Yes	No
LRP8-BFP	BFP	Yes	Yes	Diffuse
NDEL1-BFP	BFP	Yes		Diffuse
PRP-BFP	BFP	Yes	Yes	Diffuse
GAP43-GFP	GFP	Yes	Yes	Punctate
TGOLN-2-GFP	GFP	Yes	Yes	Punctate
LAMP2-RFP	RFP	No	NAe	NA
TfnR-GFP	GFP	Yes	No	NA
CgA-RFP	RFP	Yes	Yes	Punctate
SYP-BFP	BFP	Yes	Yes	Punctate
NTRK-GFP	GFP	No
Rab5a-GFP	GFP	Yes	Yes	Punctate
Rab7a-GFP	GFP	Yes	Yes	Punctate
Calreticulin-RFP	RFP	Yes	No	NA

^aAPP, amyloid precursor protein; BDNF, brain-derived neurotrophic factor; SNAP25, synaptosome-associated protein (25 kDa); VAMP1, vesicle-associated membrane protein (synaptobrevin 1); LRP8, low-density lipoprotein receptor-related protein 8; NDEL1, nuclear distribution protein nudE-like; PRP, prion protein; GAP-43, growth-associated protein; TGOLN-2, trans-Golgi network protein 2; LAMP2, lysosome-associated membrane glycoprotein 2; TfnR, transferrin receptor; CgA, chromagranin A; SYP, synaptophysin; NTRK, neurotrophic tyrosine kinase receptor type 1; Rab5a, Ras-related protein Rab-5a; Rab7a, Ras-related protein Rab-7a.

^bExpression of the fluorescent protein was assessed by infecting CAD neurons with baculoviruses and performing immunofluorescence studies.

^cTransport into axons was determined by imaging CAD neuron axons following infection with baculoviruses.

^dCAD neurons expressing these fluorescent proteins were imaged to determine whether there was punctate or diffuse fluorescence in axons.

^eNA, not applicable.