FIG 4.

RT-PCR of genes of interest. RNA was extracted from day 10 (A) and day 20 (B) raft tissues, and RT-PCR was performed to measure the transcription levels of SERPINB4, KLK8, RPTN, CDK2, and UBC. For experiments with day 10 raft tissues (A), three raft experiments, each with 16HCK and primary HCK cell lines representing three donors each, were set up. For experiments with day 20 raft tissues (B), six raft experiments, each with 16HCK and primary HCK cell lines representing three and six donors, respectively, were set up. The transcription level of the TATA-binding protein (TBP) was used as a control to normalize each measurement (statistical significance, P < 0.05, two-tailed t test). The variance of relative expression ranged from 0.0001 to 0.65 for day 10 raft tissue and from 0.0012 to 2.96 for day 20 raft tissue.