FIG 10.

Effect of E1 mutations on HCVpp infectivity. Infectivity of HCVpp harboring E1 with G311A, G315A, or W320A mutation. (A) HCVpp infectivity was determined by measuring the activity of the luciferase reporter gene in infected Huh7 cells. Pseudotyped particles produced in the absence of envelope proteins were used as negative controls. The results are reported as means ± the standard deviations (error bars) of three independent experiments. A P value of <0.05 was obtained for mutants G311A, G315A, and W320A. (B) Effect of E1 mutations on the incorporation of envelope proteins in HCVpp particles. Cells producing HCVpp were lysed and analyzed by Western blotting. HCVpps contained in the supernatants of transfected 293T cells were concentrated on a 20% sucrose cushion by ultracentrifugation and analyzed by Western blotting. E1, E2, and capsid were detected using MAbs A6, 3/11, and CRL1912, respectively.