FIG 2.

Effect of E1 mutations on the expression of viral proteins. Viral RNA transcribed from JFH1-derived mutants was electroporated into Huh-7 cells that were lysed 48 h later. Viral proteins were separated by SDS-PAGE and revealed by Western blotting with MAbs A4 (anti-E1), 3/11 (anti-E2), and anti-NS5A, as well as anti-beta-tubulin antibody, to verify loading of equal amounts of cell lysates. The protein detected by MAb A4 in cells expressing ΔE1E2 mutant corresponds to a fusion protein between the N terminus of E1 and the C terminus of E2.