FIG 5.

Effect of E1 mutations on E1E2 conformation. (A, upper panel) Interaction of HCV glycoproteins and CD81 (HCV entry factor). E1 and E2 from cell lysates were analyzed by GST pulldown at 48 h postelectroporation using a CD81-LEL-GST fusion protein. Pulled-down E1 and E2 were separated by SDS-PAGE and revealed by Western blotting with MAbs anti-E1 (A4) and anti-E2 (3/11). (A, lower panel, and B) Recognition of HCV E1 and E2 glycoproteins by conformation-sensitive anti-E1E2 MAb AR5A and anti-E1 MAb IGH526, as indicated. At 48 h postelectroporation, E1 and E2 proteins from cell lysates were analyzed by immunoprecipitation with MAbs AR5A and IGH526. Immunoprecipitated proteins were revealed by Western blotting using MAbs A4 and 3/11.