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Effect of E1 mutations on viral RNA incorporation. Huh-7 cells were electroporated with mutants and wt RNA. (A) At 48 h postelectroporation, intracellular viral RNA was extracted and quantified by quantitative RT-PCR. In parallel, the viral particles were precipitated from the supernatant with polyethylene glycol and concentrated by ultracentrifugation. (B) Extracellular viral RNA contained in the concentrated virus was extracted and quantified by quantitative RT-PCR.
