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. 2018 Sep 26;92(20):e00939-18. doi: 10.1128/JVI.00939-18

TABLE 1.

Summary of the phenotypes of E1 mutants

Wild type or mutant E1 region Infectivitya Core secretionb E1E2 heterodimerization and foldingc
 Infection inhibition assaysd
CD81 PD
 IP AR5A IP AR4A hCD81 LEL Anti-E1E2
 Anti-CD81 Anti-CLDN1 Anti-SRBI
E1 E2 AR5A AR4A
Wild type +++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++
G278A FP – – – ++ – NDe ND ND ND ND ND ND
D279A FP – – – ++ + ND ND ND ND ND ND ND
G282A FP – – ++ ++ ++ ND ND ND ND ND ND ND
L286A FP ++ – + ++ ++ ++ ++ ++ ++ ++ +++ ++
Q289A FP +++ ++ ++ ++ ++ ND ND ND ND ND ND ND
Q302A – – – ++ – ND ND ND ND ND ND ND
E303A ++ – + ++ + + ++ ++ ++ ++ +++ ++
Y309A – – – ++ – ND ND ND ND ND ND ND
G311A + ++ ++ ++ ++ ND ND ND ND ND ND ND
T314A + ++ ++ ++ ++ ND ND ND ND ND ND ND
G315A α2 + ++ ++ ++ ++ ND ND ND ND ND ND ND
H316A α2 + ++ + ++ + ND ND ND ND ND ND ND
R317A α2 +++ ++ ++ ++ ++ ND ND ND ND ND ND ND
M318A α2 – ++ ++ ++ ++ ND ND ND ND ND ND ND
W320A α2 – ++ ++ ++ ++ ND ND ND ND ND ND ND
D321A α2 – ++ ++ ++ ++ ND ND ND ND ND ND ND
M322A α2 – ++ ++ ++ ++ ND ND ND ND ND ND ND
M323A α2 ++ ++ ++ ++ ++ ++ ++ +++ +++ ++ +++ –
W326A ++ ++ ++ ++ ++ ++ ++ +++ +++ ++ ++ –
P328A +++ ++ ++ ++ ++ ++ ++ +++ +++ ++ +++ –
R339A ++ ++ ++ ++ ++ ++ + +++ +++ ++ ++ –
P341A – + ++ ++ ++ ND ND ND ND ND ND ND
a

The infectivity of HCVcc harboring the different E1E2 glycoproteins in the supernatant of electroporated Huh-7 cells was quantified 96 h postelectroporation (Fig. 3). +++, infectious titers higher than 104 FFU/ml; ++, infectious titers higher than 103 FFU/ml; +, infectious titers higher than 102 FFU/ml; −, titers between 0 and 50 FFU/ml.

b

Secretion of core Ag in the supernatant quantified at 48 h postelectroporation of Huh-7 cells (Fig. 4). ++, concentration greater than or equal to the wild type; +, concentration reduced by <1 log.; −, concentration reduced by ≥1 log.

c

The recognition of E1E2 proteins by AR4A and AR5A conformational antibodies and their interaction with hCD81 LEL were determined by precipitation experiments (Fig. 5). ++, similar amount of E1E2 precipitated to that of the wild type; +, lower amount of E1E2 precipitated; −, no E1E2 precipitated.

d

The sensitivity of the mutants to inhibition of infectivity by different antibodies or the hCD81 LEL was assessed (Fig. 6 and 7). ++, wild-type sensitivity to neutralization; +++, higher sensitivity to inhibition than the wild type; +, lower sensitivity to inhibition than the wild type.

e

ND, not determined.