FIG 5.

(A) The γ₁34.5 protein inhibits the IFN response in STING reconstituted MEFs. STING^−/− MEFs expressing h-STING were mock infected or infected with HSV-1, R3616, H1001, and H1002 (5 PFU/cell). At 5 h postinfection, total RNA extracted from cells was subjected to quantitative real-time PCR amplification for IFN-β, ISG54, ISG56, and RANTES. The results were normalized to 18S rRNA and expressed as fold activation with SDs among triplicate samples. The data were statistically analyzed by a two-tailed Student t test (**, P < 0.01). (B) Phosphorylation of IRF3. STING^−/− MEFs expressing h-STING were mock infected or infected with viruses as for panel A. Cell lysates were prepared and processed for Western blot analysis with antibodies against p-IRF3, IRF3, ICP0, ICP27, and β-actin. The data are representative of results from three independent experiments.