TABLE 2.

Primer sequences

Primer function and name	Sequencea
Y2H screen
EcoRI-UL135Y2HF	5′-GGGAATTCCATATGTCCTTATTTACCCAGCGCCGAGGCCGCAAGCGATC-3′
UL135R-BamHI	5′-CGCGGATCCTCAGGTCATCTGCATTGACTCGGCGTCCTTCATGAC-3′
pGAD-FWD	5′-TAATACGACTCACTATAGGGCGA-3′
pGAD-REV	5′-AGATGGTGCACGATGCACAG-3′
Mutagenesis
UL135ΔSH3cI-FWD	5′-PHOS-CGCCTACGCCGGCGGTCCG-3′
UL135ΔSH3c1-REV	5′-GCCGCCTCTTGCGCCCGG-3′
UL135ΔSH3cII-FWD	5′-PHOS-CCGGGGCCAAGAAGCGGCCGCCTACG-3′
UL135ΔSH3cII-REV	5′-GCGCCGGAGGTGGCTTCCAGGA-3′
UL135ΔAtypSH3-FWD	5′-PHOS-CCGCTGCAGTCGGCTGGACACCG-3′
UL135ΔAtypSH3-REV	5′-GTTTCGTGGGCGCCGGTGTTTTCT-3′
UL135ΔCIN85#1-FWD	5′-GCCGCTAAGAACCTGAGCACGCCGCCCA-3′
UL135ΔCIN85#1-REV	5′-PHOS-CGCGGGTATCGGCGTCGGGG-3′
UL135ΔCIN85#2-5-FWD	5′-GCCGCTCTGCCGCGCACCGTCGGGCTGGAGAATCTCTCGAAGGTGGGACTCTCGTGTCCCTGTCCCCGAGCCGCTACGCCGACGGAGCCGACCA-3′
UL135ΔCIN85#2-5-REV	5′-PHOS-ATTAGCGGCTGGCTTTTGTGGCGTCGGCGTTTTCGGGAAGGGAGCGGCCGTCACCGGCGGTGTCCAG-3′
Subcloning
NheI-HA-GGGG-Abi1-FWD	5′-GGGGGCTAGCACCATGTACCCATACGATGTTCCAGATTACGCGGGCGGTGGCGGTGCAGAGCTGCAGATGTTACTAGAGG-3′
Abi1-REV-XhoI	5′-GGGGCTCGAGTTAATCAGTATAGTGCATGATTGATTCAAC-3′
XbaI-CIN85-FWD	5′-ATGGTGGAGGCCATAGTGGAGTTT-3′
CIN85-HA-REV-BamHI	5′-ATGGGATCCTCAGGCGTAGTCGGGCACGTCGTAGGGGTATTTTGATTGTAGAGCTTTCTTTATG-3′
Recombinant virus construction
UL135-Fwd-63nt	5′-GTGTTTGGACAATAAACACATTCCTTGCCAAAAAATGACGTTTCCAGAAATCCAAGGCATAAATGTCCGTACACCGGCC-3′
Myc-REV-UL135	5′-GAGGGAAGGCGTGTGCTGCTATACAACTGTACAACGGACGCGCTCGCTGTTTCGGTCTCACAGATCCTCTTCTGAGATGA-3′
UL135-GalK-ins-FWD	5′-AAAAGGCGGTGCAGAGCGTCATGAAGGACGCCGAGTCAATGCAGATGACCCCTGTTGACAATTAATCATCGGCA-3′
UL135-GalK-ins-REV	5′-GTGTGCTGCTATACAACTGTACAACGGACGCGCTCGCTGTTTCGGTCTCATCAGCACTGTCCTGCTCCTT-3′
UL135-3xFLAG-FWD	5′-AAAAGGCGGTGCAGAGCGTCATGAAGGACGCCGAGTCAATGCAGATGACCGATTATAAAGATGATGATGATAAA-3′
UL135-3xFLAG-REV	5′-GTGTGCTGCTATACAACTGTACAACGGACGCGCTCGCTGTTTCGGTCTCACTTGTCGTCGTCGTCCTTGTAGTC-3′

^aPHOS, 5′ phosphorylation of oligonucleotide.