TABLE 1.
Nanomechanical and topographical parameters of T7 bacteriophage capsids and globular particles
| Parameter | Value at temp (mean ± SD) |
|||
|---|---|---|---|---|
| RT (DNA filled) | RT (empty) | 65°C | 80°C | |
| Nanomechanics | ||||
| Breaking force (F, nN) | 6.90 ± 0.97 | 1.90 ± 0.34 | 1.61 ± 0.52 | 3.83 ± 1.20 |
| Capsid stiffness (k, nm−1) | 0.73 ± 0.12 | 0.36 ± 0.10 | 0.27 ± 0.11 | 0.35 ± 0.15 |
| Maximal indentation (x, nm) | 13.24 ± 2.13 | 9.51 ± 2.41 | 12.56 ± 4.70 | 22.97 ± 6.61 |
| Capsid height (h, nm) | 60.4 ± 1.71 | 59.93 ± 1.56 | 59.41 ± 4.00 | 60.71 ± 4.87 |
| Capsid topography | ||||
| Ratio of tailed capsids | 0.83 ± 0.1 | NAa | 0.19 ± 0.12 | 0.05 ± 0.06 |
| Peak capsid height (nm) | 61.8 ± 3.6 | NA | 58.2 ± 1.7 | 63.3 ± 5.5 |
| Capsomer diameter (nm) | 11.0 ± 0.8 | NA | 9.8 ± 1.0 | 13.9 ± 2.2 |
| Globular particle parameters | ||||
| Particle height (nm) | 7.6 ± 3.2 | NA | 6.9 ± 2.5 | 10.0 ± 5.4 |
a
NA, not available.