FIG 4.

High-resolution spatial mechanical mapping of an HIV-1 core surface treated with 10 μM PF74 during reverse transcription. Cores were adhered to HMDS-coated glass slides and kept in MOPS buffer. Reactions were initiated by adding dNTPs and MgCl₂ to the cores. Images were acquired using the QI mode. (A) The mechanical map of a core before the beginning of reverse transcription. (B to F) Mechanical maps of cores after 2, 5, 7, 21, and 24 h of reverse transcription, respectively. Scale bar, 50 nm.