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Development of HIV-1 resistance to Cas9/sgPBS2. Viruses at the peaks of the first, second, and third rounds of replication in the Cas9/sgPBS2-expressing SupT1 cells were collected and sequenced for the PBS DNA. The detected indels are shown, and their frequencies are presented. The bar graphs summarize the percentages of indels involving changes of one nucleotide (1nt), two nucleotides (2nt), or three or more nucleotides (3nt).
