FIG 3.

Effect of ammonium chloride on BoHV-1 and HSV-1 entry into Vero cells. Vero cells were treated with ammonium chloride at the indicated concentrations for 20 min at 37°C. Cells were infected with BoHV-1 v4a (MOI of 2) or HSV KOS tk12 (MOI of 7.5) for 6 h in the continued presence of agents. A higher MOI for HSV-1 tk12 was required to achieve beta-galactosidase expression levels comparable to those of BoHV-1 v4a. The beta-galactosidase expression was calculated as a percentage of activity in untreated, infected cells. Cytotoxicity is shown as percent LDH activity. Values are the means from quadruplicate samples with standard deviations. Results are representative of at least three independent experiments.