Skip to main content
. 2018 Sep 26;92(20):e00592-18. doi: 10.1128/JVI.00592-18

FIG 4.

FIG 4

USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.