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. 2018 Jun 30;46(17):9160–9169. doi: 10.1093/nar/gky592

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Biosynthesis of mo⁵U by yrrM (or trmR). Presence of mo⁵U in total RNA extracted from Bacillus subtilis and the single knockout mutants was examined by triple-quadruple (QqQ) mass spectrometry in positive ion mode. Multiple reaction monitoring of mo⁵U is quantified by tracing the fragmentation of 5-methoxyuracil (mass-to-charge ratio 143.046), and an exemplary chromatogram of the parent strain is displayed (left), whereas no equivalent transition was observed in the sample prepared from ΔyrrM, or ΔtrmR.