Table 1.
Crystallographic statistics
| TrmR:tRNAAla | RNA-free TrmR | |
|---|---|---|
| Data collection | ||
| Wavelength (Å) | 0.9793 | 0.9793 |
| Space group | P 32 2 1 | P 21 21 2 |
| Cell dimensions | ||
| a, b, c (Å) | 64.98 64.98 126.68 | 76.87 116.74 43.44 |
| α, β, γ (°) | 90 90 120 | 90 90 90 |
| Resolution (Å) | 50.0–1.70 (1.73–1.70) | 50.0–2.27 (2.31–2.27) |
| R merge | 0.090 (>1) | 0.091 (0.41) |
| I/σI | 33.8 (1.5) | 20.7 (4.0) |
| CC1/2* | 0.449 | 0.976 |
| Completeness (%) | 94.1 (81.4) | 99.2 (97.6) |
| Redundancy | 17.5 (9.5) | 6.1 (5.1) |
| Refinement | ||
| Resolution (Å) | 50.0–1.70 | 30.0–2.27 |
| R work/Rfree | 0.193/0.222 | 0.216/0.266 |
| No. atoms | ||
| Protein | 1792 | 3204 |
| RNA | 317 | - |
| Ligand/ion | 32 | 54 |
| Water | 162 | 88 |
| B-factors (Å2) | ||
| Protein | 36.47 | 48.20 |
| RNA | 113.9 | - |
| Water | 45.99 | 43.11 |
| R.m.s deviations | ||
| Bond lengths (Å) | 0.009 | 0.009 |
| Bond angles (°) | 0.955 | 1.188 |
| Ramanchandran Analysis | ||
| Favored (%) | 97.66 | 95.66 |
| Allowed (%) | 1.87 | 4.34 |
| Outliers (%) | 0.47 | 0 |