Figure 4.

Analysis of SOX9 RNA- and DNA-binding. (A) SOX9 RNA-immunoprecipitation. wt and mutants (P108L, A119V, MiniSOX9, Del485) FLAG-tagged SOX9 were co-transfected with the ZDHHC16 minigene in HEK293T cells. FLAG-SOX9-wt immunoprecipitates RNA whereas FLAG-SOX9-mutants are comparable to the empty vector control condition. Enrichment of SOX9 binding sites in HT-29 cells at SOX9-dependent exon cassettes (B) and at TSS (C) of SOX9-splicing targets. A list of SOX9 enrichment peaks was obtained from the analysis of previously published ChIP-seq data from Shi Z. et al., (19). Graphs represents the specific distribution of SOX9 peaks around SOX9-dependent (red) or random (blue) exon-cassettes in C and around TSS of SOX9 splicing targets (red) or random TSS (blue) in B.