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Lower donor/recipient ratio allows increased ICEBs1 transfer in biofilm. WT donor cells were diluted and mated with a fixed number of WT recipient cells on MSgg. Transfer efficiency was measured after 20 h at 30°C. Donor/recipient ratios of 1:10 and 1:100 show significantly more ICEBs1 transfer efficiency than the 1:1 ratio (one-way ANOVA; ****, P < 0.0001). The results shown are representative of at least three independent experiments, and error bars represent the SEM.
