FIG 6.

Cell wall dynamics and inhibition of germination. (A) Spores germinated for 0, 3, 6, 12, and 24 h, stained with calcofluor white (CFW), fluorescein (FITC), and ROS stain carboxy-H₂DCFDA (ROS). (B) Germination is inhibited by 5 mM hydrogen peroxide and over 1.5 nM antimycin A, as determined by live-cell imaging, after 5 h of germination in SAB. The hydrogen peroxide control consists of an equivalent volume of H₂O, and the antimycin A control consists of an equivalent volume of 100% ethanol.