TABLE 3.

Statistics for good-quality MAGs^a

Sample type and MAG	Phylum identification	Closest relative(s)	Identification method	Coverage	% GC	% complete	% redundant	Length (Mbp)	No. of scaffolds	N50 (kbp)
Leachate
LB9	Proteobacteria	Desulfococcus oleovorans	RP16 tree	7.4	51.7	96.4	6.7	3.46	198	23.2
LB19	Bacteroidetes	LB7, LB17	RP16 tree	10.3	52.9	95.6	3.9	3.26	50	94.6
LB32	Tenericutes	LB18_1	RP16 tree	16.5	28.7	95.0	3.6	1.18	52	45.9
LB22	Firmicutes	Tepidimicrobium xylanilyticum	RP16 tree	8.1	43.3	94.2	6.2	1.58	137	12.6
LB18_1	Tenericutes	LB32	RP16 tree	26.2	33.4	92.1	3.7	1.10	75	18.6
LB10	Bacteroidetes	LB12	RP16 tree	8.0	32.8	91.3	3.0	2.31	204	12.2
LB7	Bacteroidetes	LB19, LB17	RP16 tree	99.5	42.2	88.5	5.0	2.48	223	12.5
LB27_3	CP CPR2	Uncultivated CPR2 bacteria	RP16 tree	22.2	37.5	88.2	2.2	0.74	8	17.9
LB8	Proteobacteria	Desulfotignum phosphitoxidans	RP16 tree	7.9	53.3	85.3	4.2	2.56	207	14.9
LB12	Bacteroidetes	LB10	RP16 tree	12.4	43.5	79.8	3.0	2.23	182	15.1
LB26	Firmicutes	LB26	RP16 tree	8.2	40.3	79.1	7.8	0.98	102	9.6
LB16	Firmicutes	Clostridiaceae	RP16 tree	8.9	47.0	71.9	1.9	1.85	152	15.3
LB4_2	Proteobacteria	Desulfuromusa kysingii	RP16 tree	77.4	57.6	70.7	0.8	1.13	115	10.6
River
RB10	Proteobacteria	Loktanella, RB4_2	RP16 tree	22.4	60.9	86.4	5.4	3.53	14	39.0
RB5	Proteobacteria	Simidula	RP16 tree	9.3	42.1	85.4	3.6	4.27	177	36.0
RB12	Proteobacteria	Rhodobacter	Anvi’o	29.6	64.7	71.6	4.2	3.43	33	14.5

^aGood-quality bins were defined as greater than 70% complete and less than 10% redundant. Taxonomic identification was based on a 16 ribosomal protein concatenated gene phylogeny or total gene taxonomic affiliation as calculated by Anvi’o (18). See Table S1 for statistics for all 55 leachate and 33 river MAGs.