TABLE 2.
Single nucleotide polymorphism (SNP) analysis of the C. freundii isolates carrying mutated blaKPC-2
| Isolate gene and/or gene description |
Reference nucleotide accession no. |
% of gene identity of parent to reference |
% of reads with SNP |
Nucleotide change |
Protein accession no. |
Amino acid change |
|---|---|---|---|---|---|---|
| Follow-up isolate carrying blacKPC-2-D179Y |
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| blaKPC-2 | LT992437.1 | 100 | 11 | G532T | SPM07605.1 | D179Y |
| Two-component system sensor histidine kinase EnvZ |
CP022151.1 | 100 | 100 | T434G | ASG42425.1 | V145G |
| Hypothetical protein | CP022151.1 | 99 | 100 | G101T | ASG43191.1 | G34V |
| Glyoxylate carboligase | CP022151.1 | 100 | 100 | T1292G | ASG45610.1 | V431G |
| Isolate passaged on ceftazidime-avibactam at fixed concn of 4 μg/ml of inhibitor (blaKPC-2-D176Y) |
||||||
| blaKPC-2 | LT992437.1 | 100 | 80 | G523T | SPM07605.1 | D176Y |
| Isolate passaged on ceftazidime-avibactam at 4:1 ratio (blaKPC-2-R164S + P174L) |
||||||
| blaKPC-2 | LT992437.1 | 100 | 80 | C518T | SPM07605.1 | P174L |
| LT992437.1 | 100 | 82 | C487A | SPM07605.1 | R164S | |
| Hypothetical protein | CP022142.1 | 96 | 100 | C213T | ASG77181.1 | T71M |
| 16S rRNA | CP022151.1 | 100 | 88 | T472C | ||